PREPARING SAMPLES FOR SEQUENCING
It is advisable to sequence a PCR product in both directions i.e. Forward and Reverse. But often due to financial restrictions, one direction only is sequenced. In this case it is especially important to check the quality of the sequence.
The DNA barcodes for insects book chapter details protocols for cycle sequencing and clean-up in sections 3.15, 3.16 and 3.17, but for small labs sequencing is often outsourced to local companies at increasingly competitive rates. As a rough guide you should expect to pay between US$5-10 for each sequence.
These companies will receive your PCR products (often can be sent through the mail - see the instructions by Macrogen as an example) and send back the raw sequences by email in a zip file.
It is important to label your PCR samples carefully and carefully complete the Sequencing Order Form. Our example is provided by Genomics BioSci & Tech.
Fill in the Sequencing Order Form for your PCR products.
The DNA barcodes for insects book chapter details protocols for cycle sequencing and clean-up in sections 3.15, 3.16 and 3.17, but for small labs sequencing is often outsourced to local companies at increasingly competitive rates. As a rough guide you should expect to pay between US$5-10 for each sequence.
These companies will receive your PCR products (often can be sent through the mail - see the instructions by Macrogen as an example) and send back the raw sequences by email in a zip file.
It is important to label your PCR samples carefully and carefully complete the Sequencing Order Form. Our example is provided by Genomics BioSci & Tech.
Fill in the Sequencing Order Form for your PCR products.